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. 2001 May;133(1):207–216. doi: 10.1038/sj.bjp.0704040

Figure 3.

Figure 3

(A) m3 muscarinic signalling induced by MCh (4.2×10−7 M) is not affected by relevant concentrations of QX314. Only concentrations of 10−3 M (78±8.7% of control response) and 10−2 M (55±5.1% of control response) inhibit partially. (B) Schematic illustration of the chimeras employed. (C) Concentration-response curve for effect of extracellularly applied QX314 on chimera 1. IC50 is 1.8±0.6 μM. Mean control response was 4.9±0.4 μA. (D – E) Effects of various concentrations of extracellularly administered QX314 on chimera 3 (D) and chimera 5 (E). Modest inhibition was observed in both cases (* indicates significant differences vs control). Maximal inhibition is obtained using 10−3 M QX314 on chimera 3 (64.2±7.5% of control response (1.9±0.2 μA)) and using 10−4 M QX314 on chimera 5 (58.2±8.5% of control response (1.5±0.2 μA)). (F) Extracellularly applied QX314 (10−3 M) does not inhibit chimera 2, 4 or 6. Mean response sizes are 102.1±8.5, 96.2±15 and 104.8±13.5% of control response for chimera 2, 4 and 6, respectively.