Paw-lick and jump latencies in the hot plate assay. Animals were pretreated i.p. with saline or yohimbine (Yoh) (2 mg kg−1) and treated 5 min later s.c. with saline or U-50,488 (6 mg kg−1). Animals per group were: saline n=27, U-50,488 n=29, Yoh n=7 and Yoh+U-50,488 n=7. *P<0.05 vs saline. Data are presented as mean±s.e.mean.