Figure 1.

Expression levels of forms of the CAM β₂-adrenoceptor are lower than equivalent forms of the wild-type β₂-adrenoceptor. HEK293 cells were mock transfected (1) or transfected transiently with cDNAs encoding wild-type β₂-adrenoceptor (2), β₂-adrenoceptor-Renilla luciferase (3), CAM β₂-adrenoceptor (4) or CAM β₂-adrenoceptor-Renilla luciferase (5). Forty-eight hours later membranes were prepared and the specific binding of [³H]-DHA (2 nM) measured. Data are means±s.e.mean values of triplicate assays from a representative transfection. Two further experiments produced similar results.