Figure 2.

C-terminal addition of Renilla luciferase neither inhibits constitutive activity of the CAM β₂-adrenoceptor nor the capacity of agonist to stimulate cyclic AMP production. HEK293 cells were mock transfected (1) or transfected transiently with cDNAs encoding wild-type β₂-adrenoceptor (2), β₂-adrenoceptor-Renilla luciferase (3), CAM β₂-adrenoceptor (4) or CAM β₂-adrenoceptor-Renilla luciferase (5). Twenty-four hours later cells were labelled with ³H-adenine and after a further 24 h cyclic AMP generation measured as in Methods in the absence or presence of the β-adrenoceptor agonist isoprenaline (10 μM). Results are taken from a representative experiment of three performed.