Figure 7.

Effects of ergolide on the activation of NF-κB in RAW 264.7 macrophages treated with LPS/IFN-γ. The cells were stimulated with 1 μg ml⁻¹ LPS/10 units ml⁻¹ IFN-γ for 1 h after 1 h-pretreatment of ergolide in the presence or absence of cysteine 1 mM. (A) Nuclear extracts were prepared as described under ‘Methods' and DNA-binding activity of NK-κB was determined by EMSA. (B) The nuclear extracts prepared from the experiments in (A) were analysed by Western blotting with an anti-p65 polyclonal antibody. Results show a representative blot and the mean±s.d. of the corresponding band intensities from three separate experiments. Significant differences between control and ergolide-treated groups are indicated by *P<0.05.