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Representative gels for RT–PCR products (upper panel), or amount of neuronal or inducible nitric oxide synthase (nNOS or iNOS) mRNA relative to β-actin mRNA, detected from the ventrolateral medulla under basal experimental conditions (control) or 60 min after i.v. administration of E. coli lipopolysaccharide (30 mg kg−1). Values are mean±s.e.mean of triplicate analysis, n=5–6 animals per experimental group. *P<0.05 vs control group in the Scheffé multiple-range test.
