Figure 2.

Concentration-dependence of zymosan stimulation. Cells were incubated with zymosan (0.1, 0.2, 0.3 and 0.6 mg ml⁻¹) for 18 h. (a) Effect on HO-1, iNOS and COX-2 protein expression. Results are representative of three experiments. (b) Effect on nitrite, PGE₂ and β-D-glucuronidase accumulation. Percentages were calculated with respect to the maximum level measured (67.7±2.1 ng ml⁻¹ PGE₂, 1585.8±20.6 ng ml⁻¹ nitrite and 19.9±0.9×10⁶ F.U. ml⁻¹ of β-D-glucuronidase). Data are the mean±s.e.mean of three experiments.