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Effect of LPS on the mRNA expression of IL-1β, TNF-α, IL-6 and IFNγ in activated stellate cells. (A) Cells were incubated with 1 μg ml−1 LPS for 24 h. RNA was then extracted and cDNA was prepared. Semiquantitative RT–PCR analysis was performed to determine the concentrations of mRNA for the cytokines. (B) mRNA expression of the cytokines standardized relative to the expression of β-actin mRNA. *P<0.01 vs vehicle; **P<0.05 vs vehicle.
