Figure 7.

Effect of 17-ODYA (10 μM for 75 min at room temperature) on smooth muscle hyperpolarizations induced by substance P (SP), bradykinin (BK) and NS1619 following overnight incubation of intact porcine coronary arteries in HEPES-buffered Tyrode solution (22–26 h, room temperature). During the recording, the artery segments were bathed in Tyrode solution containing 300 μM nitro-L-arginine +10 μM indomethacin. (a) Typical trace showing responses to agonists and to the bradykinin vehicle, methanol (MeOH). (b) Graphical representation of data from experiments of the types shown in (a) in which each column represents the membrane potential (m.p.),+or−s.e.mean (n=4), before and after exposure to bolus doses of substance P (1 nmol), bradykinin (0.3–1 nmol and NS1619 (330 nmol) calculated to give, transiently, the final bath concentrations indicated.