Figure 8.

Effects of overnight incubation of intact porcine coronary arteries in Krebs solution containing 10 mM HEPES (22–26 h, room temperature) on smooth muscle hyperpolarizations induced by substance P (SP), bradykinin (BK) and NS1619. During the experiment, tissues were bathed in bicarbonate-buffered Krebs solution containing 300 μM nitro-L-arginine+10 μM indomethacin+10 mM HEPES. (a) Typical trace. (b) Graphical representation of data from experiments of the types shown in (a) in which each column represents the membrane potential (m.p.),+or−s.e.mean (n=4), before and after exposure to bolus doses of substance P (1 nmol), bradykinin (0.3–1 nmol and 1-NS1619 (330 nmol) calculated to give, transiently, the final bath concentrations indicated.