Figure 1.
Dose and time-dependent expression of COX-2 by SIN-1. HUVEC were incubated for 6 h with different concentrations of SIN-1 or Molsidomine, the inactive precursor of SIN-1. COX-2 and COX-1 expression was evaluated by Western blot analysis using specific antibody for each isoform. (a) Effect of increasing concentrations of SIN-1 on COX-2 and COX-1 protein. Molsidomine, the inactive precursor of SIN-1, was tested on COX-2 expression. (b) Time-course of COX-2 induction by SIN-1. Results are representative of three different experiments.