Figure 3.

Nuclear alterations and DNA fragmentation in neurons of hippocampal neuronal/glial cultures treated with cyclosporin A. Representative micrographs show cultures exposed to 8 μM CsA for 0, 24 and 72 h; Upper panel shows CsA-treated cells with hypercondensed chromatin visualized by Hoechst 33258 staining (A, B, C), original magnification: ×200. Lower panel shows cells stained by the TUNEL staining method, typical TUNEL-positive cells indicated by arrows (D, E, F); original magnification: ×100. Cells were also stained with anti-GFAP antibody to visualize astrocytes in mixed cultures. All GFAP-positive cells are TUNEL-negative (G, H); original magnification: ×200.