Figure 5.
Effects of Aβ1–42 and Aβ42–1 on MTT reduction and neutral red (NR) uptake by primary cortical mixed cell cultures. Aβ1–42 (5, 10 and 20 μM) caused significant decreases in MTT reductions (a) as compared to the control (no Aβ peptide exposure). Aβ42–1, at 10 or 20 μM, showed no significant effect on MTT reduction. Aβ1–42, (1, 5, 10 and 20 μM) caused significant decreases in NR uptakes (b) as compared to the control. Aβ42–1, at 10 or 20 μM, had no significant effect on NR uptake by the cultured cells. Data represent mean±s.e.mean of 6–9 wells from six independent experiments. One-way ANOVA with Bonferroni's multiple comparison test and Student t-test were used to analyse statistical significance. *P<0.05; **P<0.01; ***P<0.001.