Figure 3.

Detection of superoxide level in arterioles from control, diabetic rats and diabetic rats treated with M40403. The duration of diabetes and treatments for these studies was 3 weeks. Fluorescent photomicrographs of confocal microscopic sections of arterioles that provide circulation to the region of the sciatic nerve from the three individual groups of animals were examined on the same day. Arterioles were labeled with the oxidative dye hydroethidine. Recording of fluorescent were taken at identical laser and photomultiplier settings for both control and untreated and treated diabetic rats. Shown is a representative sample of one set of animals. This experiment was repeated three separate times on separate sets of animals on three different days with similar results.