Figure 1.

Effects of 10 μM anandamide on outflow of [³H] radioactivity and longitudinal muscle contraction. The strips were stimulated twice at 0.1 Hz (10 min) (S1, S2). Anandamide was present as indicated by the horizontal bar. Upper panel: Original recording of the effect of anandamide on basal muscle tone and on electrically-evoked contractions. Note that the chart speed was increased at the beginning of the anandamide application. Lower panel: Ordinate, outflow of [³H] as percentage of the tritium present in the tissue at the start of each fraction. Mean±s.e.mean (n=4).