Figure 2.

Increase by anandamide of basal [³H]-acetylcholine release (A) and longitudinal muscle tone (B) in the absence (n=4 – 11) and presence of 1 μM capsazepine (n=4 – 6), 1 μM SR141716A (n=4 – 8), 30 nM SR144528 (n=4 – 6), or CP99994 plus SR142801 (each 0.1 μM) (n=4 – 5). The antagonists were added 59 min before anandamide. The anandamide-induced increase in basal release is given as percentage of the [³H]-acetylcholine release caused by S1, and the increase in muscle tone is given as percentage of the mean peak tension developed by all twitches during S1. Each value is the mean of n individual observations; vertical bars indicate s.e.mean. Significance of difference from the effect of anandamide alone: +P<0.05, ++P<0.01.