Figure 3.

Inhibition by anandamide of the electrically-evoked release of [³H]-acetylcholine (A) and longitudinal muscle contractions (B). Strips were stimulated twice (S1, S2) and anandamide was added 27 min before S2. The antagonists, i.e. capsazepine (1 μM), SR141716A (1 μM), SR144528 (30 nM) or CP99994 plus SR142801 (each 0.1 μM), were added 35 min before S1. Control experiments without anandamide were run in parallel (Table 1), and the effects of anandamide are given as a percentage of the corresponding control value given in Table 1. Each value is the mean of n individual observations (see legend to Figure 2); vertical bars indicate s.e.mean. Significance of inhibition of the evoked release and of twitch contraction: ^**P<0.01 (ANOVA followed by Dunnett's test). Significance of difference from the effect of anandamide alone: +P<0.05, ++P<0.01 (t-test).