Figure 4.

Effects of anandamide in the presence of tetrodotoxin on basal and electrically-evoked outflow of [³H]-acetylcholine. Electrical stimulation (0.1 Hz, 10 min) was performed 9 min (S1) and 78 min (S2) after the end of the washout period. Horizontal bars indicate superfusion with tetrodotoxin (TTX) and TTX plus anandamide. Control experiments, effect of a 18 min superfusion with tetrodotoxin (TTX) on the electrically-evoked outflow of [³H]-acetylcholine during S2. The S2/S1 ratio (0.89±0.06, n=4) was not different from that in experiments without TTX (see Table 1). Anandamide (n=4) was superfused first together with TTX (9 min), and then without TTX.