Figure 1.

Time course of LPS-stimulated NFκB-DNA binding and IκB degradation in RASMC. Serum starved RASMC were exposed to vehicle (C) or 100 μg ml⁻¹ LPS (L) for the times indicated. NF-κB-DNA binding (A), IκB-α, (B), IκB-β (C) and IκB-ε (D) protein expression were measured as described in the Methods section. The autoradiographs and blots shown are representative of three individual experiments.