Figure 5.

Effect of H₂O₂ on LPS-stimulated IKK/NF-κB signalling Serum starved cells were pretreated with vehicle (C) or increasing concentrations (as indicated) of H₂O₂ (H) for 30 min prior to exposure to 100 μg ml⁻¹ LPS (L). NF-κB-DNA binding (A), protein expression of IκB-α (B), and IκB-ε (C) and kinase activity of IKK-α (D) and IKK-β (E) were measured as described in the Methods section. The autoradiographs and blots shown are representative of three individual experiments.