Figure 4.

Aβ(25 – 35)-induced neuronal cell death and generation of eicosanoids from Neurons. Cortical neurons were treated with 10 μM Aβ(25 – 35). MTT-reducing activity (A) and eicosanoids (B) were measured at the indicated time points after Aβ treatment. PGD₂ (circles), PG E₂ (triangles) and LBT₄ (squares) were measured with their RIA kits. The control level of PGD₂, PGE₂ or LTB₄ was 73±3, 198±20 or 752±38 pg/ml, respectively. Data are expressed as mean±s.e.mean values (n=4). **P<0.01, compared with vehicle control by ANOVA followed by Dunnett's test. Vehicle control is treated with culture medium containing 1% deionized water and 0.1% DMSO.