Figure 3.

Effects of the anti-VLA-4 (HP2/1, 10 μg ml⁻¹), anti-Mac-1 (ICRF 44, 1 : 12 diln) monoclonal antibodies and a non-specific control monoclonal antibody (mAb, 10 μg ml⁻¹) upon human eosinophil adhesion after treatment with L-NAME, 1.0 mM, (a) to 20 μg ml⁻¹ fibronectin, (b) to 10% (v v⁻¹) human serum. Eosinophils (>95% pure) were incubated with monoclonal antibodies (+L-NAME) (25 min, 37°C, 5% CO₂) before allowing cells to adhere to fibronectin/serum-coated plates (15 min, 37°C, 5% CO₂). Results are expressed as mean adhered cell percentages of total cell number±s.e. of four independent experiments with three replicates in each. +P<0.05 compared to untreated cells, **P<0.01, ***P<0.001 compared to cells treated with L-NAME alone (1.0 mM).