Figure 2.

Effects of Dan, GIF-0082, and GIF-0185 on Ca²⁺ release. (a) In intact fibres, after treatment with dantrolene (Dan), GIF-0082, or GIF-0185 in the concentration range of 0.2 – 50 μM, the size of twitch contraction was compared with that before the treatment. Relative tensions were plotted against the concentrations. (mean±s.e.mean, n=9 for Dan, n=3 – 6 for GIF-0082, n=3 – 6 for GIF-0185). ^*P<0.05 (Dunnett's test). (b) The rates of Ca²⁺ release were obtained in saponin-skinned fibres with 20 μM Dan (n=5), 50 μM GIF-0082 (n=3 – 7) or 50 μM GIF-0185 (n=3 – 4) in the absence of Mg²⁺ and adenine nucleotide at pCa>8 – 4.5. Open triangles show the rates without these agents (control, n=11 – 16). The symbols for GIF-0082 and GIF-0185 were slightly offset horizontally for clarity (mean±s.e.mean). ^*P<0.05, ^**P<0.01 (vs control). Error bars smaller than the symbols are not shown.