Figure 4.

Different effect of GIF-0082 and GIF-0185 on Ca²⁺ release. (a) In skinned fibres, the rates of Ca²⁺ release measured with Dan (0 – 50 μM) at pCa 6.0 under Mg²⁺-free conditions were normalized to the control value (without Dan) obtained in the same preparation in the absence (Dan alone, mean±s.e.mean, n=3 – 6) or presence of 50 μM GIF-0082 (n=3) or GIF-0185 (n=5). (b) The rates of Ca²⁺ release by 10 mM Clof in the presence of Dan (2 or 5 μM, mean±s.e.mean) and/or either GIF-0185 (5 μM) or GIF-0082 (25 μM, n=4) under Ca²⁺, Mg²⁺-free conditions (buffered with 10 mM EGTA) were normalized to the control value (without these agents) within each preparation. ^*P<0.05, ^**P<0.01.