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Detection of endostatin protein by immunoprecipitation and Western blotting in rat platelet lysates. Immunoreactive endostatin from rat platelets shows a 20 kDa band similar to mouse endostatin. Lane A: 5 ng of mouse endostatin standard (from Chemicon International); Lanes B – D: Immunoprecipitate of rat platelet endostatin from 500, 1000 and 2000 μg of total platelet lysate protein; Lane E: Immunoprecipitate of 10 ng of mouse endostatin standard.
