Figure 5.

Effects of O-desulphated heparin on HPBMNC adhesion to HUVECs under different experimental conditions. HUVECs were stimulated with (a) IL-1β (100 u ml⁻¹), (b) TNF-α (1000 u ml⁻¹) or (c) LPS (100 μg ml⁻¹) for 6 h, in the absence or presence of O-desulphated heparin (ODSH). ⁵¹Cr-labelled HPBMNCs were added for 30 min, either alone, to HUVECs stimulated in the presence of ODSH (open bars) or, in conjunction with different concentrations of ODSH, to HUVECs stimulated in the absence of the drug (closed bars). Adhesion was quantified by γ-counting lysates of adherent cells and was calculated as a percentage of that seen in control wells that had not been treated with ODSH. Data are expressed as the mean±s.e.mean of six separate experiments, each performed in triplicate. ^*Statistical significance (P<0.05) is indicated.