Figure 2.

Effects of the treatment with rolipram or anti-TNF-α on the increase in vascular permeability and recruitment of neutrophils in the intestine (A,B) and lung (C,D) following severe ischaemia (120 min) and reperfusion (120 min) of the superior mesenteric artery. Changes in vascular permeability (A,C) were assessed by evaluating the extravasation of Evans blue dye and neutrophil recruitment (B,D) was assessed by evaluating the tissue levels of myeloperoxidase. Rolipram (10 mg kg⁻¹) was given s.c. in two equally divided doses 60 and 15 min prior to reperfusion and anti-TNF-α antiserum (5 μl g⁻¹) was given s.c. 60 min prior to reperfusion. Control animals (Vehicle) received phosphate buffered saline or control sheep serum (results in control animals are pooled for presentation). Results are shown as μg Evans blue or as number of neutrophils per 100 mg of tissue and are the mean±s.e.mean of five animals in each group. ^*P<0.01 when compared to sham-operated animals and #P<0.01 when compared to mild I/R animals.