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. 2001 Nov;134(5):1093–1103. doi: 10.1038/sj.bjp.0704342

Figure 5.

Figure 5

Effects of aloe-emodin and emodin on the release of cytochrome c and the expression of caspase-3 and PARP in CH27 and H460 cells. The effect of aloe-emodin and emodin on cytochrome c (Cyt. c), caspase-3 (Cas-3) and PARP was detected by Western blot analysis in CH27 (A) and H460 (B) cells. Cells were incubated with 40 μM aloe-emodin or 50 μM emodin in the presence of 1% serum for 2, 4, 8, 16 and 24 h. Cell lysates were analysed by 8% (PARP), 12% (caspase-3) and 15% (cytochrome c) SDS – PAGE, and then probed with primary antibody as described in Materials and methods. Results are representative of three independent experiments.