Figure 1.
Expression of the adenosine receptors and β-actin transcripts in A375 cells. The PCR product in lane 2 is 394 bp long according to the human A1 receptor sequence, that in lane 3 is 208 bp long according to the human A2A receptor sequence, that in lane 4 is 153 bp long according to the human A2B receptor sequence and that in lane 5 is 156 bp long according to the human A3 receptor sequence. Lane 6 is 654 bp long according to the human β-actin sequence. Lanes 1 and 8 are molecular weight markers. Total RNA was extracted as described in Methods and used for RT – PCR (lanes 2 – 6) or directly used for PCR to detect β-actin DNA contaminant sequences (lane 7). Ten μl of PCR product were loaded in each lane. RT – PCR products obtained from A375 cells and separated on a 2% agarose gel.