Figure 3.

Effects of prostaglandin receptor antagonists on PGD₂- and PGE₂-induced CGRP release. (A) Cultured trigeminal ganglion neurones were incubated with release buffer for 30 min and then subsequently challenged with 1 μM PGD₂, PGE₂ or cPGI₂ with or without the DP receptor antagonist, BWA 868C (1 μM). Co-incubation wells were pre-incubated with antagonist for 30 min before being incubated with the appropriate test agonist. *P<0.05 against PGD₂ alone. (B) Rat trigeminal ganglion neurones (4 – 6 days in culture) were incubated with either release buffer for 30 min and then PGE₂ alone or the EP₁ receptor antagonist, ZM325802, (both 1 μM) for another 30 min or ZM325802 (1 μM) for 30 min and then PGE₂ and ZM325802 (both 1 μM) for 30 min. Data are presented as the percentage increase in CGRP release over baseline and represent the mean±s.e.mean of 3 – 5 independent experiments. ^***P<0.001 vs control.