Figure 2.

VEGF-induced activation of PLCγ. (A) Western blot analysis of VEGF time-dependent effect on PLCγ activation. Near-confluent BAEC were serum-starved in DMEM for 16 h and stimulated with VEGF (1 nM) for various periods of time. Cells were lysed, proteins were immunoprecipitated with an anti-PLCγ antibody and separated with a 10% SDS – PAGE. Western blot analysis was performed with an anti-PY20 antibody. (B) and (C) effect of U73122 (10 μM) on VEGF- and PBS-induced PLCγ phosphorylation respectively. BAEC were pretreated 10 min with U73122 and U73343 prior to a 5 min VEGF treatment.