Figure 3.

VEGF effect on p42/44 MAPK phosphorylation. (A) Western blot analysis of VEGF time-dependent effect on p42/44 MAPK activation. Confluent BAEC were stimulated with VEGF (1 nM) for various periods of time. Cells were lysed, proteins were separated on a 10% SDS – PAGE. Western blot analysis was performed with an anti-phospho-p42/44 MAPK antibody. (B) BAEC were pretreated with genistein (Gen, 10 μM), U73122 (10 μM), GF109203X (GFX, 1 μM), PD98059 (PD, 10 μM), SB203580 (SB, 10 μM), then the cells were treated as in (A).