Figure 9.

VEGF effect on IP production. (A) Confluent BAEC were labelled with [³H]-myoinositol, and stimulated with or without VEGF (1 nM) for various periods of time. The cells were scraped, total IP were isolated by extraction with 1,1,2-trichlorotrifluoroethane and tri-n-octylamine, purified by chromatography with a Dowex AG1X8 column (200 – 400 mesh) and quantified by scintillation counting. The values are means of at least four experiments±s.e.mean. (B) Confluent BAEC were pretreated either with U73122 (10 μM), U73343 (10 μM), Wortmannin (Wort; 100 nM) or LY294002 (LY; 10 μM), and stimulated with VEGF (1 nM) for 5 min. IP production was determined as in A. ^***P<0.001 as compared with control buffer (PBS). †††P<0.001 as compared with VEGF (1 nM) as determined by analysis of variance followed by a Bonferoni t-test.