Localization of MIP-1α and MCP-1 in the granulomatous tissue. Cryostat sections (10 μm) showing (A) MIP-1α staining in fibroblasts (arrow) and (B) in MNC (likely to be macrophages, arrows). (C) Negative control of pre-absorbed MCP-1 polyclonal antibody on paraffin embedded sections (3 μm). (D) Immunoreactivity for MCP-1 in fibroblasts and mast cells localized mainly in dermis and some scattered mast cells in the granuloma layer. (E) High magnification of fibroblasts and MNC cells MCP-1 positive detected in dermis in the rectangular field in (D). (F) High magnification of mast cells MCP-1 positive observed in dermis layer indicated by the arrowhead in (D). Bar, 20 μm for panels A, B, E and F; bar, 50 μm panel D and 100 μm panel C.