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. 2001 Dec;134(7):1429–1436. doi: 10.1038/sj.bjp.0704381

Figure 6.

Figure 6

NFPS is an apparent irreversible inhibitor. (a) Representative current trace from an oocyte expressing GLYT1b voltage clamped at −60 mV. After a control (10 μM) glycine response, glycine (10 μM) was reapplied until the current reached a maximal response (point 1) followed by the co-application of 300 nM NFPS. Three minutes later NFPS was washed out of the bath, but in the continued presence of 10 μM glycine (point 2). After washout of glycine for a further 5 min, 10 μM glycine was reapplied (point 3). Currents induced by subsequent application of 10 μM glycine up to 90 min later remained equally and significantly inhibited (Kruskal-Wallis test). (b) Current responses to application of 10 μM glycine were measured at the indicated points (1 – 3) and represented in a bar graph (n=6). Significance (P⩽0.05) is indicated by *.