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Butyrate increased Fas and Fas L levels. MCF-7 cells were treated with 2.5 mM butyrate for different periods of time. Proteins from whole-cell extracts, membrane, and cytosolic preparations (100 μg) were electrophoresed, and Western blots were performed as described in Methods. The experiment was repeated 4 times. The loading and transfer of equal amounts of protein were confirmed by immunodetection of actin.
