Figure 5.

Western blot analysis of P53, Bcl-2, and Bax in MCF-7 and T47-D cells. Pre-confluent cells were treated with 2.5 mM butyrate for different periods of time. Proteins (20 μg) were electrophoresed and Western blots were performed as described in Methods. The experiment was repeated three times. The loading and transfer of equal amounts of protein were confirmed by immunodetection of actin.