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Butyrate-induced apoptosis was blocked by anti-Fas antagonist antibody. MCF-7 cells were treated with 2.5 mM butyrate in the presence or absence of 25, 50, or 100 ng ml−1 Fas antagonist antibody for 48 h. Apoptosis was determined after Hoechst staining. The Figure shows the results of a triplicate assay in one experiment, which is representative of three independent experiments.
