Figure 3.

Effect of 2APB (100 μM) on thapsigargin (1 μM)-induced changes in intracellular Ca²⁺. In the absence of extracellular Ca²⁺ thapsigargin induced a transient increase in Ca²⁺_i; subsequent addition of Ca²⁺ to the superfusate resulted in a large increase in Ca²⁺_i (a; n=5). (b) Illustrates that 2APB while not preventing the initial thapsigargin-induced increase in Ca²⁺ prevented subsequent capacitative Ca²⁺ entry (n=5). Washout of 2APB restored entry of Ca²⁺. Results are presented as mean±s.e.mean. For clarity, representative error bars are shown.