Figure 6.

Effects of cytoskeletal disruption on ATP-induced changes in intracellular Ca²⁺. Cells were exposed to cytochalasin for 1 h at 37°C in a tissue culture incubator. As treatment did not cause a significant change in baseline flourescence ratio between groups, data are presented as normalized to per cent change from baseline prior to ATP stimulation. Exposure of cells to cytochalasin D (100 nM or 1 μM) had no apparent effect on the initial ATP-induced Ca²⁺ release while significantly inhibiting the capacitative Ca²⁺ entry phase (n=6 for each condition). Results are presented as mean±s.e.mean. For clarity, representative error bars are shown.