Cross-sectional view of a 3-D reconstructed isolated vascular smooth muscle cell from arterial (A to D) and venous origin (E to J). This figure illustrates the intracellular calcium distribution of VSM cells isolated from arteries in response to IRL-1620 (1 μM; B,C,F and G) (A: control; B: 5 min; C: 10 min) or ET-1 (0.1 μM) (D: 10 min) and VSM cells isolated from veins (E: control; F: 5 min; G: 10 min; H: KCl, 30 s, I: EGTA, 5 min; J: Syto: 11). The white scalebar represents length in μm. The colour scale represents pseudocolor, intensity levels of Fluo-3 from 0–255 and Ca concentrations from 0.01 to 40 μM. This figure is representative of three other experiments.