Figure 1.

Time course of PAH uptake in hOAT1-transformed and vector-transformed OK cells. The hOAT1-transformed (closed circles) and vector-transformed control (open circles) cells were incubated with 200 μl of Dulbecco's PBS containing 5 mM D-glucose, 10 μM D-[³H]-mannitol and 10 μM [¹⁴C]-PAH for various time periods at room temperature. Radioactivity in the cell lysate was measured. Extracellular trapping and nonspecific uptake were obtained from the radioactivity of nontransportable D-[³H]-mannitol. Values are mean±s.d. (n=4).