Figure 7.

Effects of uraemic toxins on cellular free radical production of hOAT1-transformed OK cells. The hOAT1-transformed cells (closed columns) or vector-transformed control cells (open columns) were incubated in Dulbecco's PBS containing 5 mM D-glucose, 20 μM dihydrofluorescein diacetate and indicated amount of uraemic toxins for 60 min at 37°C, and fluorescence intensities were measured. Values are mean±s.d. (n=4). (A) Indoxyl sulphate. (B) Indoleacetic acid. (C) Hippuric acid. (D) p-hydroxyhippuric acid. (E) o-hydroxyhippuric acid. *P<0.05 vs vector-transformed cells. †P<0.05 vs non-treated control.