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. 2002 Jan;135(2):555–563. doi: 10.1038/sj.bjp.0704482

Figure 9.

Figure 9

Characteristics of indoxyl sulphate-induced cellular free radical production. (A) Effects of anti-oxidants on indoxyl sulphate-induced cellular free radical production. The hOAT1-transformed cells (closed columns) or vector-transformed control cells (open columns) were incubated in Dulbecco's PBS containing 2 mM indoxyl sulphate, 5 mM D-glucose, 20 μM dihydrofluorescein diacetate and indicated amount of anti-oxidants for 60 min at 37°C, and fluorescence intensities were measured. Values are mean±s.d. (n=4). *P<0.05 vs vector-transformed cells. †P<0.05 vs 2 mM indoxyl sulphate-treated cells. (B) Effects of transporter inhibitor on indoxyl sulphate induced cellular free radical production. The hOAT1-transformed cells (closed columns) or control cells (open columns) were incubated in Dulbecco's PBS containing 2 mM indoxyl sulphate, 5 mM D-glucose, 20 μM dihydrofluorescein diacetate and indicated amount of probenecid for 60 min at 37°C, and fluorescence intensities were measured. Values are mean±s.d. (n=4). *P<0.05 vs vector-transformed cells. †P<0.05 vs 2 mM indoxyl sulphate-treated cells.