Figure 4.

Dependence of CCE on the thapsigargin exposure time. After cells were treated with 1 μM thapsigargin (TG) for 1.5 min (trace a), 2 min (trace b), 3 min (trace c), 5 min (trace d), 7 min (trace e), or 10 min (trace f), as indicated, extracellular Ca²⁺ was removed, then, 1 min later, the ATP (100 μM) was added. [Ca²⁺]_i increase was measured. The experiment was repeated six times using different batches of cells with similar results.