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. 2002 Mar;135(6):1403–1414. doi: 10.1038/sj.bjp.0704610

Figure 2.

Figure 2

Inward currents induced by cholinergic agents. Current-recordings were made at a holding potential (VH) of −40 mV. (A) The inward currents induced by various concentrations (3×10−8 – 10−5 M) of ACh. The hyperpolarizing step pulses from a VH of −40 mV to −55 mV with 500 ms duration were applied every 15 s. (B) The inward currents induced by nicotine (3×10−6 – 3×10−4 M). (C) The inward currents elicited by muscarine (10−7 – 3×10−6 M) and oxotremorine-M (OX-M, 3×10−8 – 10−6 M). (D) Effect of atropine on the ACh response. Four consecutive responses to ACh (10−7 or 10−5 M) in the absence or presence of atropine (3×10−7 M). ACh was applied for 1 min (10−7 M) or 30 s (10−5 M) every 6 min. It is noted that the rapid component of ACh (10−5 M)-induced current was clearly observed when the agonist was washed out. The figure is representative of four reproducible experiments.