Involvement of the M1 receptor in muscarinic response. Recordings were made at a VH of −40 mV. Aa, Inward currents induced by OX-M (IOX-M) in the presence of pirenzepine (3×10−7 M), AF-DX116 (3×10−6 M) or Z-338 (3×10−5 M). Ab, Effect of various concentration of pirenzepine, AF-DX116 and Z-338 on IOX-M. Antagonists were applied 1 min before application of OX-M. Ba, Inhibitory effects of MT-7 (5×10−8 M) on OX-M (3×10−7 M)-induced inward currents. MT-7 was applied 3 min before application of OX-M. Each column shows the average of five cells, and vertical bars indicate s.e.mean.