Figure 5.

Effects of ADA inhibitor CF and nucleoside transporter inhibitor NBMPR on RA – FLSs apoptosis. RA – FLSs were incubated for 24 h in triplicate with the indicated reagents, after which DNA fragmentation was quantitated by ELISA. NBMPR partially inhibited the DNA fragmentation elicited by 2-CADO. Adenosine did not induce apoptosis even in the presence of both CF and NBMPR. Representative results from five independent experiments are shown. The data are normalized to the values obtained in the absence of 2-CADO and Ado, which was arbitrarily assigned a value of 1.0; #, P<0.05 vs control incubation.