Figure 6.

Effect of 10 min treatment with 30 μM U50,488H on the subcellular distribution of PKC-α (A) and -ε (B) in ventricular myocytes pretreated with 1 μM U50,488H for 24 h. Upper panel: Representative Western blots. Lanes 1 and 2 represent, respectively to cytosolic fractions isolated from untreated and U50,488H-treated cells. Lanes 3 and 4 represent, respectively to particulate fractions isolated from untreated and U50,488H-treated cells. Lower panel: Group results showing expression of different isoforms as assessed by densitometric analysis. Data are expressed as percentage changes in the density of autoradiographic bands of cytosolic (C) or particulate (P) fractions from cells subjected to 30 μM U50,488H (hatched bars) relative to those from untreated cells (white bars, 100%). The data are expressed as mean±s.e. (n=6). *P<0.05 vs corresponding control.