Figure 1.

Oleamide enhanced the effects of both glycine and GABA at their respective receptors. (a) Perfusion of increasing concentrations of oleamide to oocytes responding to the EC₂₀ of Glycine and GABA. Control responses are shown on the far left of each trace. (b) Concentration-response curves for oleamide modulation of glycine and GABA_A receptors (Glycine: Max=1.72±0.34, EC₅₀=22.12±1.4, Hillslope=1.9) (GABA: Max=2.16±0.35, EC₅₀=28.94±1.4, Hillslope=2.4, n=4 – 11). (c) Compounded data from replicated experiments. Unpaired, 2-tailed Student's t-tests were used to compare the maximum responses, EC₅₀s and hillslopes for the two curves. Only the EC₅₀ was significantly different (P=0.305, 0.017, 0.781 respectively, n=4 – 11).